Urea, Urease, Cyanate, and the Sickling of Hemoglobin S
نویسندگان
چکیده
منابع مشابه
Urea, urease, cyanate, and the sickling of hemoglobin S.
Urea inglucose solutions has been advanced as a chemotherapeutic agent in sickle cell disease because it has been found effective both in reversing and in blocking sickling. It has been suggested recently that this beneficial action of urea may be the result of formation of cyanate from urea and subsequent carbamylation of beta S globin chains in the hemoglobin S molecule. In this paper, we sho...
متن کاملAutomated dithionite test for rapid, inexpensive detection of hemoglobin S and non-S sickling hemoglobinopathies.
Automated adaptations of dithionite and urea-dithionite tube tests are accurate, reliable, inexpensive methods for detecting hemoglobin S. More than 3,000 individuals have been screened (120 determinations per hour; reagent cost, 2 to 4 cents per test). The dithionite reagent consists of potassium phosphate, sodium dithionite, and saponin. When S hemoglobin contacts this reagent, the red cells ...
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We examined the extent to which the intracellular polymerization of sickle hemoglobin (HbS) can account for the severity of anemia and of vaso-occlusive manifestations in the various sickling syndromes. Polymer formation in sickle cell disease depends principally on the intraerythrocytic hemoglobin composition and concentration. In our studies, the polymer fraction in sickle red cells was deter...
متن کاملSpot test for detection of sickling hemoglobin.
We describe a simple, noninstrumental procedure for detecting hemoglobin S in samples of blood on filter paper, with use of small volumes of reagents. The method depends on the differential solubilities of reduced sickling and nonsickling hemoglobin in phosphate solutions (1, 2) or Sickledex reagent’ and the apparent solubilizing effect of urea on reduced hemoglobin S (3). The color of paired p...
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Many studies of the kinetics of enzyme action as a function of temperature have been made. The older work (for reference cf. Haldane, 1930; Tauber, 1937) indicates that while the velocity of an enzyme-catalyzed reaction increases with rise in temperature, up to the inactivation temperature of the enzyme, this change in rate is not an exponential function of the absolute temperature. I t has bee...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1972
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/18.9.961